صفحه 1:
= | سيد
BIO-SURFACTANT
Zahra Tavakkol
صفحه 2:
WHAT IS SURFACTANT ?
Surfactant :Surface active agent
+ lower the surface tension between :
1)two liquids
2)liquid and a solid
3)Liquid and air
hydrophil hydrophob
ON
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Classification
hydrophil hydrophob
a> OC SS
آذآ سکس 2۳
Bee
2۳ 3 سس
(1) Classification by structure |
(© RCOONa
ionic 1 cationic - 6 9
L amphoteric =O) R-NH,*CH,CH,COO
ester R-COO(CH,CH,O),H R-COOCH,C(CH,OH),
ether R-O(CH,CH,O),H: Perregal
anionic
*\ nonionic 1 RN CHCR Oe
amine R-NQ(CH,CH,O),H
5 (0را یا رز
amide RCON{ (cy,CH,0),H
algal _—_— R-(OCH,CH,),0S0,Na*
nonionic-anionic
http://
hosnani.com/
surfactants.php
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ها
HLB
Solubiizing agents (15-18)
Hydrophilic | Detergents (13-15)
(water soluble)
‘ow Emutsifying agents (8-16)
Wetting and
Water 4
ی | spreading agents (7-9)
| \wlo Emulsitying agents (3-6)
Hydrophobic Antifoaming agents
(oil solutie) | 88
https://en.wikipedia.org/
wiki/Hydrophilic-
lipophilic balance
The hydrophilic-lipophilic
balance
Griffin’s method.
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Critical micelle
concentration
Surface tension (mN/m)
Biosurfactant concentration (g/L)
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FUNCTION
Roll-up
Surfactants may act as detergents, wetting agents,
emulsifiers, foaming agents, and dispersants. ۶
5 1
| NS t 422 تا
هدس
3
SY
کر
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Infant respiratory distress
syndrome (IRDS)
a a و نس
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SP-C Structure
Jehanmaon, eta, 1994 Biochemicoy 33, 6015
http://
www.t
relazioni/
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Bio-surfactant ?
surface-active substances synthesized by living cells.
Can be made from : plant oil, plant carbohydrate and animal oil
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SS cn ™
BIO-SURFACTANT VS.
SU RFACTAN
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4
[BIOTECHNOLOGY - Vol V— Production of Bissutictans -F. Sirs RK Hommel, H-P. Kleber
PRODUCTION OF BIOSURFACTANTS, oo
Biosurfactant Tension
5 (mN m*)
[Cellobioselipids F, Sineriz 5 <1
اه سوم Institute of Microbiology, University of Tucuman- PROIMI, Argentina 1-18
Mannosylerythriol
lipids R. K. Hommel 1
Rhamnoselipids Cell Technologie Leipzig Germany 22
Sophoroselipids تون Lo
وه ی Institute of Biochemistry, University of Leipzig, Germany
mycolates
mono | non-ionic 32 3 16
1 non-ionic 36 4 17
1 non-ionic 26 15 1
Lipopeptides licheniformis glucose of non-ionic 27 0.02-10 1
Surfactatin Bacillus subtle 3۹ ef on fonle 2 5 1
Deb, = eell-bound; c.f. = cell-free (extracellular)
in dependence on culture conditions
Table 1. Some properties of selected biosurfactants from a range of microorganisms.
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HYSIOLOGICAL ROLE OF BIO-SURFACTANT IN
MICROORGANISMS
* permit microorganisms to grow on water-immiscible substrates
* antimicrobial activities towards various micro-organisms
* emulsify the hydrocarbon substrate and facilitate its transport into the cells
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FACTORS AFFECTING BIO-SURFACTANT
PRODUCTION
Carbon sources: Diesel, crude oil, glucose, sucrose, glycerol
Nitrogen sources: urea peptone, yeast extract, ammonium sulphate,
ammonium nitrate, sodium nitrate, meat extract and malt extracts
Environmental factors: temperature, pH, aeration
Salt concentration
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TYPES OF BIO-SURFACTANT
Biosurfactants are mainly classified according to
their chemical structure and their microbial origin.
1)glycolipids,
2)phospholipids,
3)polymeric biosurfactants and lipopeptides (surfactin).
Sophorolipids J] sophorose
Rhamnolipids [rhamnose
Trehaloselipids [trehalose
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* Glycolipids : Rhamnolipids , trehalolipids , sophorolipids
0 Rhamnolipids :
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TYPES OF BIO-SURFACTANT
* Glycolipids : Rhamnolipids , Trehalolipids , Sophorolipids
0 Trehalolipid : °
0
po
mycolates group : He Ho
Mycobacterium, Ho 2
Rhodococcus, none
Arthrobacter, 0
Nocardia 9
Gordonia[8] 6
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TYPES OF BIO-SURFACTANT
+ Glycolipids : Rhamnolipids , trehalolipids , sophorolipids
0 Sophorolipids : sophorose
0
09
candida and the starmerella ZA
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۱۸0000
8
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|
peptide loop of seven
amino acids (L-aspartic
acid, L-leucine,
glutamic acid, L-
leucine, L-valine and
two D-leucines)
TYPES OF BIO-SURFACTANT
+ Lipopeptides and lipoprote
Bacillus subtilis ] Surfactin | hemolytic acti
wy جر هه
WARY se
hydrophobic fatty acid chain thirteen to fifteen
carbons long which allows it to penetrate cellular]
membranes.
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ها
TYPES OF ۳ ۲
+ Lichenysin
Bacillus licheniform. 0 NH جم 0 ‘oh
5 9 ۳
۴ OX
Ho’ Pact HO ی
Lichenysin A Surfactin
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۱
APPLICATIONS
Biosurfactants have several applications in agriculture, medicine, petroleum
and industry.
Deepwater Horizon
Detergents, Emulsions , Soaps , Paints , Adhesives , Inks , Anti-fogs
Biopesticide
Agrochemical formulations
+ Herbicides
* Insecticides
In medicine : Antimicrobial , Anticancer , Antiviral , gene delivary , drug
delivery, Immunological adjuvants
food processing industry
Application of biosurfactant in petroleum
Cosmetics
* Shampoos
+ Toothpastes
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|
The overall biosurfactants market is estimated to rise at a
compound annual growth rate of 5.60 percent between 2017 and
2022
* Sophorolipid is the most important type of the overall
biosurfactants market
* Detergents is the most important application segment of the
overall biosurfactants market
+ Europe (EU) to be the biggest biosurfactants market during the
outlook period
https://www.reportlinker.com/p05253739/Biosurfactants-Market-by-Type-
Lipopeptides-Phospholipids-Polymeric-Biosurfactants-Application-And-Region-
Global-Forecast-to.html
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م۳۳
<<
HOME ABOUT AGAE APPLICATIONS —RE5.——-RNAMNOLIDGISHURFACTANT هم
ORDER —_MAYLU PRODUCTS NTACT US
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۱
REFERENCES
+ Franzetti, A., Gandolfi, |., Bestetti, G., Smyth, T. J. P. and Banat, |. M.
(2010), Production and applications of trehalose lipid biosurfactants. Eur.
J. Lipid Sci. Technol., 112: 617-627. doi:10.1002/ejlt.200900162
+ Santos DKF, Rufino RD, Luna JM, Santos VA, Sarubbo LA. Biosurfactants:
Multifunctional Biomolecules of the 21st Century. Taubert A,
ed. International Journal of Molecular Sciences. 2016;17(3):401.
doi:10.3390/ijms17030401.
* Fakruddin Md (2012) Biosurfactant: Production and Application. J Pet
Environ Biotechnol 3:124. doi:10.4172/2157-7463.1000124
صفحه 25:
۳ | یت
Thanks for your attention