میانکنش نانوذرات با بیومولکول‌ها

میانکنش نانوذرات با بیومولکول‌ها

اسلاید 1: Biomolecules–Nanoparticles: Interaction in Nanoscale Presentation : Sajjad BabaeiPhD student of Nanobiotechnoogy. Razi University. Kermanshah. Iran1

اسلاید 2: IntroductionNanomaterials that measure 1–1,000 nm allow unique interaction with biological systems at the molecular level.As nanoparticles and biomolecules are of similar length scale, it seems logical that the combination of biomacromolecules to nanomaterials can provide interesting tool for mimicking the biomolecules which are present at cellular systems, probing the mechanisms of biological processes, as well as developing chemical means by handling and manipulating biological components.2

اسلاید 3: The interactions of biomolecules and metal nanoparticles arise that determine the size of nanoparticles, modify the surface of nanoparticles to enhance solubility/biocompatibility/biorecognition, and detoxification of toxic metals.the macromolecule and nanoparticles interaction helped in ultra-trace detection, imaging, biomolecules detection, drug and DNA/RNA delivery, cancer therapy, and photodynamic therapy.3

اسلاید 4: The different groups of macromolecules that interact with nanoparticles4

اسلاید 5: The interaction of nanoparticles with nucleic acids and proteins are very high due to the presence of active functional groups on the surface; while that of carbohydrates and lipids is comparatively lower.5

اسلاید 6: Nucleic Acid–Nanoparticles InteractionAmong the various nanoparticles, gold followed by silver and platinum are highly explored for use in various molecular level applications due to their high affinity with nucleic acids.nanoparticles are highly sensitive spectroscopic reporters for the base-pairing of DNA.Aptasensors Aptamer : Aptamers are oligonucleotide or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool.6

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اسلاید 10: Protein–Nanoparticles InteractionNanoparticles are capable of strong and important interaction with other molecules.Gold nanoparticle as metal based beads, have specific importance due to their attractive physical and chemical properties, biocompatibility, and facile surface modification.In general, nanoparticles have the ability to interact with whole physiological surrounding once when they enter human body. In most of the cases, first molecule they interact with are proteins, which are the main constituens of human body and the driving force of most of the biological processes.10

اسلاید 11: Size of nanoparticles makes them able to enter in almost all parts of human body, including cells and organelles, while flat surfaces can only affect biological processes via cell surface receptors such as integrins.When nanoparticles enter biological fluid, the first molecule that will react with nanoparticles, are proteins in more than 95% of all cases.The result of protein coating on nanoparticles surface is protein corona.Protein corona may influence cellular uptake, inflammation, accumulation, degradation and clearance of nanoparticles11