Basic Immunologic Proceduresروشهای اساسی ایمونولوژیک

Basic Immunologic Proceduresروشهای اساسی ایمونولوژیک

اسلاید 1: Basic Immunologic Procedures روشهای اساسی ایمونولوژیک

اسلاید 2: IntroductionDetection of antigen/antibody reactions difficultCan measure EITHER antigen or antibody.Sensitization is the binding of a specific antibody to its’ specific antigenSensitization cannot be visualizedMultitude of laboratory methods have been developed to make this reaction visible

اسلاید 3: Factors Which Affect ReactionsConcentrations of reactantsTemperatureLength of incubationpH of test system

اسلاید 4: Three Distinct Phases of Antigen/Antibody ReactionsPrimary Phenomenon – SensitizationSecondary Phenomenon – Lattice formationTertiary Phenomenon – Detected by affect on tissues or cells.

اسلاید 5: Primary phenomenon Sensitization – binding of antibody to antigen – not visible

اسلاید 6: Primary phenomenonSensitization – binding of single antibody to single antigen siteThese tests are Difficult ComplexExpensiveRequire special equipmentTime consuming

اسلاید 7: Primary Phenomenon TestsTechniques include:ImmunofluorescenceRadioimmunoassayEnzyme immunoassay