صفحه 1:
صفحه 2:
Introduction
Osterton of cotyed/catbody reusioos dPPicut
9 Ova were CIDER catgen pr uitbody.
o Gevetizaioa te the brody oP a speviie oatbody tr ts’ spevPic
من
لح ها اوه ماوق ۲
جكا حادم صا حارط موی جرم| عون روت سل Oultude oP 11
reunion visible
صفحه 3:
Factors Which Affect
Reactions
۲ و سل و00 reacts
00
ولمم ۴و یمسر تا
PW oF test spstew
صفحه 4:
Antigen/Antibody
Reactions
۲ Ortivey Phewweowd — Orustizdiva
۲7 Gppowdey Pkewweow — butice Porto
۲7 Deroy Pkewweud — Ortevied by oPPevt va tesurs
0
صفحه 5:
Primary phenomenon
۲1 Geestizaios — brady oF cotbody to calged — wt visible
Orn <<
i
/
۱ و
5 ye
صفحه 6:
Primary phenomenon
Gevstizuiog — biodoy of stage vebody to stage voles
ie
۲ ۲۳ tess or
= OFPod
™ Ovwplex
۶ مسق
® Require spevid equiped
© Dive اص ورین
صفحه 7:
Primary Phenomenon
Tests
صفحه 8:
Secondary Phenomenon
a Geveiizaiod tokeo o step Purther to batice Porwotioc
& ab oP @utbody wolevue biads to two seporde wie
فرط رم
۶ 1۳ چيه من مه structures suk us R@Os onses
ری
© 4P bots cotbody ved wotged ae svluble resus ta previpicica
صفحه 9:
Secondary Phenomenon
Reactions
صفحه 10:
Secondary Phenomenon
۲ Dhese tests are!
® Guy w موم
® Less expewsue
۲ Less toe poosurvicy
1" Op wt require ppevid equpwedt
۲ چا و0
® Less sowie
© Less spevhc
1" Oore tterPerewe
صفحه 11:
Secondary Phenomenon
© Gxawples oP tests:
® سس
® Oxghutcaiod
1" Cpwpewerd Praia
صفحه 12:
Tertiary Phenomenon
9 Reunion wt visible, detected by PP ect oP rewiod vo
fesues vr velb.
© Deets techie:
« مطد خی
® @kxpoposs
© Oceposiion oP kowuce cowplexe>
® Chowune wherewe
® Chewornts
1 دكا عدب اعدعد. جسذادداعد. سس / :ها
صفحه 13:
Phagocytosis
Macrophage “Aniibody
\, Antigenic
determinant
صفحه 14:
Secondary Phenomena
Most Frequently Utilized
° @revipratca — svluble watbody rewsts wih اوه سارت
© Oygfutcaicg — particude cetgeos boued together by watbody
Poke visible cocoplex
© Cowplewedt Prratza — ueibody birdy مت و ۲
و مرش powplewedt, resus tr vel pote
صفحه 15:
Antigen-Antibody
Binding
a QOuve oP cotged vad catbody requires
a رم
= ری
۲ 6۳۳ ved widiy deterwied by baw oP Ouse Orica
صفحه 16:
Antibody Affinity
۲7 Oesorbes the stead: oP a stage Oxy-Ob bed.
5 @s @y acd Ob cows close toyether o chewiod bood
Pons whick is wedk ood poo dissociate.
© dbow well the @b Pits the By wil detercotur stubiliy oP
bod, “lock ood hep” Al hoe sirogest oP Pray.
۲ )9 ww rew! wik srocturdy stra xp, resus in
DrDss rewiiy.
© Qopst walbodies have u high oPPiaiy Por their ان
صفحه 17:
Low Affinity
Affinity
High Affinity
صفحه 18:
Avidity
۲ Oeserbes te vowbied اجه oP wubiple Oxy-Bb brad.
5 4Aettdly bood te cusiy broke, but wuliple biadoge of the scree
the dssvctaics & pvercowe by the sheer ancber oF bods
۳
9 @uidiy ts tePlusared by bots he vdeare oP the ootbody ued te
deus oP te ooied.
صفحه 19:
۰
106 109
Avidity Avidity
10٩
Affinity
Ky
صفحه 20:
Affinity versus Affinity
© OP Rely rePers to the streaghs oF birdy betwerd a
sige wolqruo detercotcact ced oc tedividudl catbody
powbidtey ste wheres wily rePers to te pverd _
sireohy oP biodedy betvero wulivdeo coligeds ud
اه
صفحه 21:
IgM and IgG
ع الحاصل صو مور Oost Prequedly detevted ©
۲ 10 ۳ زرا oP Paty but high aidip due to 40 بلج
Bie.
صفحه 22:
Law of Mass Action
13 Gowers the reversblliy oP the cotqec-udtbody reaios.
۲ RQeverstle rewion, visble reaction cours wheo the rote oF
brediog exceeds the اس نس
© Os 42 بقلت لحن رود teoreuses, sreughedw reais.
Ag+ Ab <> Ag-Ab
Applying the Law of Mass Action
IAg-Ab]
[Ag] x [Ab]
4
صفحه 23:
Precipitation Curve
9 Oepeads va powedruiod oP By aad ۰
۲ م۳ — wabody excess, wary wbodes vod ul
oiqed sites- resus ta Puse vequive
8 @ostove — vwolged excess, wolbody cous voiged bu
رم( | اي بووین resus to Poe رش
۲ و ور Cquidews — vottged ud catbody presect tc
ppitod proporiives to bred cod que visible reaction
صفحه 24:
gat | هو
oo) ey
Ab excess Ag excess
Equivalence - Lattice formation
صفحه 25:
Precipitation Curve
Rg ee
ve
صفحه 26:
Precipitation Curve
0 ۳ 0 5
2
موه
N
Antigen added
Antbody-excess Equivalence
zone zone
excess Ab)
Supernatant الس
‘Antibody preciptated