صفحه 1:
صفحه 2:
Word/Terms List ۳
Agglutinin -
a
Equivalence zone ٠
۲۱۵۸ ۰
Immunodiffusion -
Immunoelectrophoresis -
RIA ۰
۱۱:23
صفحه 3:
Affinity ۳
¢ Strength of the reaction between a single
antigenic determinant and a single Ab
ae ۳
High
۱۳
۱۰۱۰۱۱۱۱۱۱۵۱۱۱۱۱ ار
forces
صفحه 4:
Specificity ۳
aa (eet) i ٠ ۰۱۱۱۵ عط
antibody combining site to react
یت ریم یر یه ۱
٠ 6ه late عط
antibody molecules to react with
معوأغمة عمه بزلمه.
صفحه 5:
Cross Reactivity ۳
¢ The ability of an individual Ab combining
site to react with more than one antigenic
determinant.
* The ability of a population of Ab
EG Cross reactions
Shared 1600م تتملتسزة
epitope
صفحه 6:
Factors Alrecting
Measurement of Ag/Ab
Reactions
»ومد py
٠١ Affinity 4
«۲ Ab excess | Ag excess
٠ Ag:Ab ratio و دي
* Physical form of A 2
Equivalence - Lattice formatip: اش 1
صفحه 7:
Tests Based on Ag/Ab ۳
Reactions
۶ عطه2ع۲ ۸9/۵۵ (ه 0عععه عاعع] للد
will have to depend on lattice
مغ عفتاقط ااانا بإقطغ عه ممأغهصمم؟
utilize ways to detect small
immune complexes
۶ عطهز2ع۲ ۸۵0/و۸ ده 0ععوه عاعه] اام۸
can be used to detect either Ag or
Ab
صفحه 8:
Agglutination Tests ۳
Lattice Formation
صفحه 9:
Agglutination/ ۳
Hemagglutination
* Definition - tests that have as their
endpoint the agglutination of a
particulate antigen
tA US Re ا
Ag or Ab -
ل 7 7 2
صفحه 10:
د
512
>2
3*2 5 58
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000
دهع حالس
3
ای
‘sod
۳/1
تقرف
569/1
كفن
2۸:
ce/T
91/1
وف
۸۶
cL
أسمعنهمط
1a انا
Prozone >
6 نا
Hemagglutination
Quantitative aggluti
Agglutination/
صفحه 11:
Agglutination/ ۳
Hemagglutination
Definition >
Qualitative test >
Quantitative test >
° Applications
- Blood typing
Sei CuR ا
-Fourfold rise in tit
© © © © 2
© © © © 8
* Practical considerations
- Easy
- Semi-quantitative
صفحه 12:
Passive
Agglutination/Hemagglutin
۴ ۷:۲۱ 0006 9۶ع۲ ۵:09]باووه الع اتنا
a soluble antigen coated onto a particle
° Applications
- Measurement of antibodies to soluble
antigens
صفحه 13:
رز
Inhibition
PTC eC Ml dC a
agglutination due to competition with a soluble Ag
Prior to Test
Y + #۳ 1 ل
A
© © 15
ی
١0 oy عامسده 5د أسععوط
صفحه 14:
رز
Inhibition
Definition
Applications >
Measurement of soluble Ag >
وتا ریاد هو i
Same as agglutination test >
صفحه 15:
Precipitation Tests 8
LATTICE FORMATION
صفحه 16:
Radial Immunodiffusion
ار ۲۰۲۱۱۲۵/۰۱
Method
ات
۰۲۱۱۱ للدت
۱۳۲۵۲۵۲۵۲۵۲۱۵۵ le
Diameter of ring >
ا
مهاعم مععمم فطع
Quantitative >
Ig levels >
1
2
oy
E
a
صفحه 17:
Method Le
Ags are separated by electrophoresis >
Ea ccetn tit يا
- Precipitin arc represent individual antigens
صفحه 18:
ene es oe
Method >
Interpretation >
Qualitative >
مماغو ف مععمم عمبالغواعم
صفحه 19:
an مر نارازه اهنا
Enzyme-Linked
Immunosorbent Assays (EIA)
LATTICE FORMATION NOT
REQUIRED
صفحه 20:
a
Yre a ¥
Labeled
و4
ا ا
sample ۳
rior to Test
تلكا
a
Competitive RIA/ELISA for وم
Ltt) >
Determine amount >»
را وتات را ارت
PC MCE ltl Lig
of labeled Ag
- Use
predetermined
amounts of
labeled Ag and
Ab and add a
sample
containing
unlabeled Aq as
صفحه 21:
Solid Phase Reece مانام م
RIA/ELISA
Ab detection > |
Immobilize Ag » ۹۳۹
ا ا Patient’s| |
sample Br تال ye
Add labeled anti-Ig > Immobilized}
Amount of labeled >
Ab bound is
proportional to
Telia e-}
sample
صفحه 22:
1
Ab
70
sample \ @
Ag in
Patient’
1۳0012۵81
Solid Phase Non-Competitive ۳
VL YN
Ag detection >
Immobilize Ab >
Incubate with sample >
Add labeled antibody >
Amount of labeled Ab>
bound is proportional
to the amount of Ag
in the sample
صفحه 23:
Tests for Cell Associated
Antigens
LATTICE FORMATION NOT REQUIRED
صفحه 24:
Immunofluorescence | |
* Direct
- Ab to tissue Ag is labeled with
fluorochrome
صفحه 25:
Immunofluorescence ۳
Indirect >
۱9 *واز و۸ عبووز] مغ طلم
x, 1
۳:۹ سد 0 unlabeled
Fluorochrome-labeled >
۱
anti-Ig is used to ۳
detect binding of the ۳
-first Ab ل
* Qualitative to Semi-
Quantitative
صفحه 26:
Assays Based on | |
Complement
LATTICE FORMATION NOT REQUIRED
صفحه 27:
Complement Fixation 0
“طم عه a eo Lt Ne طاغأير لععاتد وم
Standard amount of complement is added -
۱۳ AMAR ا ال ل ا
را ONO Ce CA cerita
اننا
Ratti’