صفحه 1:
صفحه 2:
A Comparative
Phytochemical
and Physicochemical
Evaluation:
Tikta-anguna, Kiri-anguna
and Jeewanti
صفحه 3:
ملس
Obevives
Oxterids ved Ortkods
Results
صفحه 4:
Introduct
ion
Tikta-anguna and Kiri-anguna (Behet anguna)
Both come under one botanical name -
Wattakaka volubilis (Linn.f)
Family: Asclepiadaceae
+ Both creepers are large twining shrubs, with
long glabrous branches, Leaves thin, ovate
1. Bitter variety
2. Sweet variety
صفحه 5:
a (@ehot corps)
Dis cere conn
صفحه 6:
Parts Used:
Leaves, roots and tender stalks
are used
Actions and common uses:
Leaves are used for boils and abscesses as
an application
Roots and tender stalks are used for
expectorant properties in Ayurveda and
traditional medicine
Lactogenic
صفحه 7:
Jeewanti
Leptadenia reticulate (Retz) Wight
and Arn.
صفحه 8:
L. reticukie
+ Parts Used:
Both roots and leaves
Actions and common uses:
Both roots and leaves - Skin infections,
wounds
Leaves - cough, as a respiratory stimulant,
Lactogenic
Aphrodisiac
Cooling and Tonic
صفحه 9:
Research background : Ayurvedic texts
contain lot of simple and effective as well as
both externally and internally used medicinal
preparations, containing Jeewanti (L.
reticulata)
Jeewanti is found in most parts of India, but
it is not available in Sri Lanka
We had to find a most suitable substitute for
Jeewanti
صفحه 10:
* According to literature survey and the
conversation with Senior Scientist in
charge, Haldummulla Research
Gardens, we were able to _ find
morphologically, nearly similar two
plants (Tikta-anguna and Kiri-anguna)
for Jeewanti
* In addition, they have similar
practices for disease conditions
صفحه 11:
Comparison of the Two plants L. reticulate
and W. volubilis
W. volubilis
Asclepiadaceae
Hemajivanti,
Sujivanti,
Svarnajivantika
Many similarities
Skin ailments
Respiratory tract
infections
ENT disorders
Lactogenic
Burning sensation
Antipyretic
Use for poisoning 11
Aphrodisiac
L.
oe
Asclepiadaceae
Jivanti,
Svarnajivanti,
Tittajivanti
Many similarities
Skin ailments
Respiratory tract
infections
ENT disorders
Lactogenic
Burning sensation
Antipyretic
Use for poisoning
Aphrodisiac
Family
Classical
names
Morphology
Common
uses
صفحه 12:
Purpose of this study was to
investigate the possibility of using
Tikta-anguna and Kiri-anguna as
substitutes for Jeewanti
صفحه 13:
Objectiv
a To compare the phytochemical parameters of
W. volubilis (Tikta-anguna and Kiri-anguna) and
L. reticulata (Jeewanti)
Qa To compare the physicochemical parameters
of
W. volubilis (Tikta-anguna and Kiri-anguna) and
L. reticulata (Jeewanti)
OTo compare the fingerprint profiles of W. volubilis
(Tikta-anguna and Kiri-anguna) and L. reticulata
(Jeewanti)
صفحه 14:
Materials and
Methods
W. volubilis (both varieties) were collected from
Colombo, Sri Lanka
Authentication was done by Scientist , Botany
Division, BMARI, Navinna, Maharagama
Voucher specimen No: W 2561
L. reticulata was collected from Jamnagar, India
Authentication was done by Botanist, Vidyaratnam
Foundation (Research and Development Section),
Thrissur, Kerala, India, according to the standards of
Ayurveda Pharmacopeia in India
صفحه 15:
Plant materials
* Plant materials (leaves with tender stalk)
were shade dried and cut into small pieces
Then prepared the powder using the grinder
+ Materials were kept in air tight containers
until used
صفحه 16:
Screening of Phytochemical
compounds
* Presence /Absence of
* Phenolic compounds
Flavanoids
Tannins
* Coumarins
* Alkaloids
* Saponins
* Steroid glycosides
صفحه 17:
* Determination of
physicochemical parameters
Physicochemical parameters
were evaluated according to WHO
standards
صفحه 18:
Crdtroctuble «utter tc
Hot Water Extract (HWE)
Hot Ethanol Extract (HEE)
Cold Water Extract (CWE)
Cold Ethanol Extract (CEE)
% Extractable mater = Weight of extract
x 100
Weight of sample
18
صفحه 19:
@ Ash Content :
1. % Total ash = Ash Weight
x 100 TT
Weight of sample
2.% Acid-insoluble ash = Acid insoluble Ash
Weight x 100
Weight of
sample
3. % Water-soluble ash
صفحه 20:
TLC fingerprint profiles of W. volubilis
and L. reticulata
* Extract - methanol extract
* Plant material - 4.0 g
* Extracts were spotted on a silica gel plate and
TLC fingerprints were developed using
Dichloromethane: Ethyl acetate: Cyclohexane
(40:1:10 v/v/v)
* Vanillin-sulphuric acid reagent was used for
colour development
صفحه 21:
Statistical
analysis
* Data were analyzed by using Mann
Whitney test and findings of p<0.05
was considered to indicate statistical
significance
٠ All data were presented as mean = SEM
- All the values were express as dry
weight of the sample and they were
performed in triplicate for accuracy
صفحه 22:
Results
Table 1 : Phytochemical classes of W. volubilis varities
and L. reticulata
Phytochemi Presence or Absence of Phytochemicals
cal Classes
Classes Tikta-anguna _|Kiri-anguna Jeewanti
(Behet-anguna)
عم | عم | (ewe) | (CEE) | aw | cee | ccwe) | (CEE) | awe) | cHEE | ewe) | (CEE
داه 9 | > >
لا | لا | | لا | لا | لا لا ااال Phenolic
compounds
+ | | | لب | | له | | | | | له Saponins
Flavanoids اله - - - vi - - - - - - -
صفحه 23:
Screening of phytochemical compounds of
(a) cold and hot ethanol and water extracts of ۰
volubilis and
L. reticulata revealed the presence of phenolic
compounds, tannins, and steroids
(b) cold and hot water extracts of W. volubilis and L.
reticulata revealed the presence of saponins
(c) cold and hot ethanol and water extracts of L.
reticulata and hot extracts of W. volubilis revealed the
presence of coumarin
(d) In ad
flavanoids
ion, hot water extract of W. volubilis contained
صفحه 24:
Table 2: Physicochemical parameters of Wattakaka
L. Reticulata
(%)
(Dry wt basis)
Geewanti)
31.54 + 0.46
13.19 +0.42*
5.99 + 0.49
6.34+ 0.13*
reticulata
W. Volubilis (%) - (Dry
wt basis)
Tikta Kiri anguna
anguna (Benet
anguna)
37.374 31.67 +
0.56 0.33
19.62+ | 3.36 + 0.05
0.10
7.99+ 7.01+ 0.28
0.04
1.55+ 1.37 + 0.05
volubilis and Leptadenia
Physico-chemical
parameters
Hot water extractable
matter
Hot ethanol extractable
matter
Cold water extractable
matter
Cold ethanol extractable
۳
صفحه 25:
Colowtey tree physicochewiod paraweters of مرول
were siqaPicndy dPPereat Pro thot oP Tikto-caqued ood (Geeooquad
0. Wot ord Ookd Croc @xtractuble wutter
9. Tord wk Orceut
3. @okHesvhible wh Crue
25
صفحه 26:
9: PLO Pragerpriat proPies oP Mite-cecucd, (treooguad cod lero
سم
1
0 9 9 1 9 9
و ره تشه ow ou ow
Per spray
ات لت شین
Oar buk— 1 OOF owe
retkta (leewoct ) با ,0
(مسجممب) حطس .0 .9
(مسمممسها١) اظطاص .0 :6
صفحه 27:
sup
LO- decstiograw Preerprict oP Tikte-coquea, رتسول له میم
8۵۵ - Tiktaeoguna (W.
volubilis)
Blue - Kiri anguna (W.
volubilis)
Green = Jeewanti (L.
صفحه 28:
TLC Pragerprivts oP 0 بجلسسو)
رتسول له تممپبممب) لك
لول were 77 2
three sawples
صفحه 29:
According to the results of this study,
phytochemical parameters,
physicochemical parameters and
fingerprint profiles of Tikta-anguna and
Kiri-anguna have very similar properties
and they are similar to Jeewanti also
29
صفحه 30:
conclusions
* As this study reveals, Tikta-anguna, Kiri-anguna and
Jeewanti have more similarities in terms of phytochemical
parameters, physicochemical parameters and fingerprint
profiles
+ Therefore, both Tikta-anguna and Kiri-anguna can be used
as substitutes for Jeewanti in ayurvedic preparations
صفحه 31:
According to traditional medicine, Kiri-anguna (Behet-
anguna) is commonly used for medicinal preparations
Further, Kiri-anguna (behet-anguna) can be
considered as the “sweet variety” of W. volubilis
and it has similar properties such as GUNA (Quality),
RASA (Taste), VIPAK (Metabolism), VIRYA (Potency),
PRABHAV (Impact) to Jeewanti
After considering all, we can conclude that ki
anguna (behet-anguna) is much better to use as a
substitute for Jeewanti
31
صفحه 32:
Referenc
es
Parangepe, P., Indian medicinal Plants, 2005, Chaukhamba Sanskrit
Pratishthan, Delhi.pp 116-117
Jayaweera, D.M.A., Medicinal Plants (Indigenous and Exotic) Used in
Ceylon, 2006, The National Science Foundation, Sri Lanka, pp 225
Kirtikar, K. R., Basu, B. D., Indian Medicinal Plants- volume Ill,
1996,Valley offset printers and publishers, Dehra Dun, pp 2267-2268
Senaratna, L. K., A Check List of Flowering Plants of Sri Lanka, 2001,
The National Science Foundation, Sri Lanka, pp 46
Sharma, P.C., Yelne, M.B., Dennis, T.J., Database on Medicinal plants
used in Ayurveda, 2002, Central Council for Research in Ayurveda
and Siddha, New Delhi. Pp 270-272
32
صفحه 33:
Acknowledgem
ent
Mr Piyal Marasingha - Senior scientist ,
Dept of Ayurveda
Dr. Sudeesh Kumar and the Vidyaratnam
Foundation (Research and Development
Section), Thrissur, Kerala, India
Industrial Technology Institute
National Centre for Advanced Studies for
Humanities and Social Sciences (NCAS)
صفحه 34:
Thank you
صفحه 35:
» Jeewanti Tikta-anguna
Kiri-anguna (Bitter verity)
(Sweet verity)
Rasa Madhura Katu, Tikta Madhura
Guna Laghu, Snigdha Ruksha, Laghu
Laghu, ......
Veeryasheeta Ushna Sheeta
Vipaka Madhura Katu Madhura
Prabhawa Jivniya