A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti

A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti

اسلاید 1: 1www.tabaye.ir

اسلاید 2: A Comparative Phytochemical and Physicochemical Evaluation: Tikta-anguna, Kiri-anguna and Jeewanti 2

اسلاید 3: IntroductionObjectivesMaterials and MethodsResultsConclusion3

اسلاید 4: Tikta-anguna and Kiri-anguna (Behet anguna)Both come under one botanical name - Wattakaka volubilis (Linn.f)Family: AsclepiadaceaeBoth creepers are large twining shrubs, with long glabrous branches, Leaves thin, ovate1. Bitter variety2. Sweet varietyIntroduction4

اسلاید 5: Wattakaka volubilisKiri anguna (Behet anguna)Tikta anguna5

اسلاید 6: Parts Used:Leaves, roots and tender stalks are used Actions and common uses:Leaves are used for boils and abscesses as an applicationRoots and tender stalks are used for expectorant properties in Ayurveda and traditional medicine LactogenicAphrodisiacSweet variety –cooling and Tonic6

اسلاید 7: Jeewanti Leptadenia reticulate (Retz) Wight and Arn.7 Family : AsclepiadaceaeA much - branched twining shrub, branches numerous and younger ones glabrous, Leaves thin, ovate

اسلاید 8: Parts Used:Both roots and leaves Actions and common uses: Both roots and leaves - Skin infections, wounds Leaves - cough, as a respiratory stimulant, LactogenicAphrodisiacCooling and TonicL. reticulate8

اسلاید 9: Research background : Ayurvedic texts contain lot of simple and effective as well as both externally and internally used medicinal preparations, containing Jeewanti (L. reticulata) Jeewanti is found in most parts of India, but it is not available in Sri Lanka We had to find a most suitable substitute for Jeewanti 9

اسلاید 10: According to literature survey and the conversation with Senior Scientist in charge, Haldummulla Research Gardens, we were able to find morphologically, nearly similar two plants (Tikta-anguna and Kiri-anguna) for Jeewanti In addition, they have similar practices for disease conditions10

اسلاید 11: Comparison of the Two plants L. reticulate and W. volubilis L. reticulate W. volubilis FamilyAsclepiadaceaeAsclepiadaceaeClassical namesJivanti, Svarnajivanti, TittajivantiHemajivanti, Sujivanti, SvarnajivantikaMorphology Many similaritiesMany similaritiesCommon usesSkin ailmentsRespiratory tract infectionsENT disordersLactogenicBurning sensationAntipyreticUse for poisoningAphrodisiacIncrease urinationSkin ailmentsRespiratory tract infectionsENT disordersLactogenicBurning sensationAntipyreticUse for poisoningAphrodisiacIncrease urination11

اسلاید 12: Purpose of this study was to investigate the possibility of using Tikta-anguna and Kiri-anguna as substitutes for Jeewanti 12

اسلاید 13: ObjectivesTo compare the phytochemical parameters of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti)To compare the physicochemical parameters of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti)To compare the fingerprint profiles of W. volubilis (Tikta-anguna and Kiri-anguna) and L. reticulata (Jeewanti)13

اسلاید 14: Materials and MethodsW. volubilis (both varieties) were collected from Colombo, Sri Lanka Authentication was done by Scientist , Botany Division, BMARI, Navinna, Maharagama Voucher specimen No : W 2561L. reticulata was collected from Jamnagar, IndiaAuthentication was done by Botanist, Vidyaratnam Foundation (Research and Development Section), Thrissur, Kerala, India, according to the standards of Ayurveda Pharmacopeia in India14

اسلاید 15: Plant materialsPlant materials (leaves with tender stalk) were shade dried and cut into small pieces Then prepared the powder using the grinder Materials were kept in air tight containers until used 15

اسلاید 16: Screening of Phytochemical compounds Presence /Absence of Phenolic compounds Flavanoids TanninsCoumarinsAlkaloidsSaponinsSteroid glycosides16

اسلاید 17: Determination of physicochemical parameters Physicochemical parameters were evaluated according to WHO standards 17

اسلاید 18: Hot Water Extract (HWE) Hot Ethanol Extract (HEE) Cold Water Extract (CWE) Cold Ethanol Extract (CEE) % Extractable mater = Weight of extract x 100 Weight of sample18Extractable matter in

اسلاید 19: Ash Content : 1. % Total ash = Ash Weight x 100 Weight of sample 2. % Acid-insoluble ash = Acid insoluble Ash Weight x 100 Weight of sample 3. % Water-soluble ash = Total Ash Weight - water insoluble residue in total ash x 100 Weight of sample19

اسلاید 20: TLC fingerprint profiles of W. volubilis and L. reticulataExtract - methanol extractPlant material - 4.0 gExtracts were spotted on a silica gel plate and TLC fingerprints were developed using Dichloromethane: Ethyl acetate: Cyclohexane (40:1:10 v/v/v) Vanillin-sulphuric acid reagent was used for colour development20

اسلاید 21: Statistical analysisData were analyzed by using Mann Whitney test and findings of p<0.05 was considered to indicate statistical significanceAll data were presented as mean ± SEMAll the values were express as dry weight of the sample and they were performed in triplicate for accuracy21

اسلاید 22: Table 1 : Phytochemical classes of W. volubilis varities and L. reticulataPhytochemical ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPresence or Absence of Phytochemicals ClassesPhytochemical ClassesTikta-angunaTikta-angunaTikta-angunaTikta-angunaKiri-anguna (Behet-anguna)Kiri-anguna (Behet-anguna)Kiri-anguna (Behet-anguna)Kiri-anguna (Behet-anguna)JeewantiJeewantiJeewantiJeewantiPhytochemical Classes(HWE)(HEE)(CWE)(CEE)(HWE)(HEE)(CWE)(CEE)(HWE)(HEE)(CWE)(CEE)Phenolic compoundsSaponins------Flavanoids----------Steroid glycosidesTanninsCoumarin----Alkaloids---------22Results

اسلاید 23: 23Screening of phytochemical compounds of (a) cold and hot ethanol and water extracts of W. volubilis and L. reticulata revealed the presence of phenolic compounds, tannins, and steroids (b) cold and hot water extracts of W. volubilis and L. reticulata revealed the presence of saponins (c) cold and hot ethanol and water extracts of L. reticulata and hot extracts of W. volubilis revealed the presence of coumarin (d) In addition, hot water extract of W. volubilis contained flavanoids

اسلاید 24: Physico-chemical parametersW. Volubilis (%) - (Dry wt basis)W. Volubilis (%) - (Dry wt basis)L. Reticulata (%) (Dry wt basis) (Jeewanti)Physico-chemical parametersTikta angunaKiri anguna (Behet anguna)L. Reticulata (%) (Dry wt basis) (Jeewanti)Hot water extractable matter37.37± 0.5631.67 ± 0.3331.54 ± 0.46Hot ethanol extractable matter19.62± 0.103.36 ± 0.0513.19 ±0.42*Cold water extractable matter7.99± 0.047.01± 0.285.99 ± 0.49Cold ethanol extractable matter1.55± 0.021.37 ± 0.056.34± 0.13*Total ash content11.80± 0.0716.46± 0.1116.63 ± 0.30*Acid-insoluble ash Content0.91± 0.020.81± 0.012.30 ± 0.02*Water-soluble ash Content5.76± 0.216.11 ± 0.066.13 ± 0.03Table 2: Physicochemical parameters of Wattakaka volubilis and Leptadenia reticulata24

اسلاید 25: Following three physicochemical parameters of Jeewanti were significantly different from that of Tikta-anguna and Kiri-anguna1. Hot and Cold Ethanol Extractable matter2. Total ash Content3. Acid-insoluble ash Content 25

اسلاید 26: L. reticulata (Jeewanti ) 2. W. volubilis (Kiri-anguna)3. W. volubilis (Tikta-anguna) 1 2 3 2µL 2µL 2µL 1 2 3 2µL 2µL 2µLWave length – at 254 nm After spraying Vanillin -sulphuric acid3. TLC fingerprint profiles of Tikta-anguna, Kiri-anguna and Jeewanti26

اسلاید 27: Red – Tikta anguna (W. volubilis) Blue – Kiri anguna (W. volubilis) Green – Jeewanti (L. reticulata) TLC- densitogram fingerprint of Tikta-anguna, Kiri-anguna and Jeewanti, at 254 nm27

اسلاید 28: According to TLC fingerprints of Tikta-anguna, Kiri-anguna and Jeewanti, similar compounds were identified in three samples28

اسلاید 29: According to the results of this study, phytochemical parameters, physicochemical parameters and fingerprint profiles of Tikta-anguna and Kiri-anguna have very similar properties and they are similar to Jeewanti also29

اسلاید 30: conclusionsAs this study reveals, Tikta-anguna, Kiri-anguna and Jeewanti have more similarities in terms of phytochemical parameters, physicochemical parameters and fingerprint profilesTherefore, both Tikta-anguna and Kiri-anguna can be used as substitutes for Jeewanti in ayurvedic preparations30

اسلاید 31: According to traditional medicine, Kiri-anguna (Behet-anguna) is commonly used for medicinal preparations Further, Kiri-anguna (behet-anguna) can be considered as the “sweet variety” of W. volubilis and it has similar properties such as GUNA (Quality), RASA (Taste), VIPAK (Metabolism), VIRYA (Potency), PRABHAV (Impact) to JeewantiAfter considering all, we can conclude that kiri-anguna (behet-anguna) is much better to use as a substitute for Jeewanti 31

اسلاید 32: ReferencesParangepe, P., Indian medicinal Plants, 2005, Chaukhamba Sanskrit Pratishthan, Delhi.pp 116-117Jayaweera, D.M.A., Medicinal Plants (Indigenous and Exotic) Used in Ceylon, 2006, The National Science Foundation, Sri Lanka, pp 225Kirtikar, K. R., Basu, B. D., Indian Medicinal Plants- volume III, 1996,Valley offset printers and publishers, Dehra Dun, pp 2267-2268Senaratna, L. K., A Check List of Flowering Plants of Sri Lanka, 2001, The National Science Foundation, Sri Lanka, pp 46Sharma, P.C., Yelne, M.B., Dennis, T.J., Database on Medicinal plants used in Ayurveda, 2002, Central Council for Research in Ayurveda and Siddha, New Delhi. Pp 270-27232

اسلاید 33: AcknowledgementMr Piyal Marasingha – Senior scientist , Dept of AyurvedaDr. Sudeesh Kumar and the Vidyaratnam Foundation (Research and Development Section), Thrissur, Kerala, IndiaIndustrial Technology Institute National Centre for Advanced Studies for Humanities and Social Sciences (NCAS)33

اسلاید 34: Thank you34

اسلاید 35: JeewantiTikta-angunaKiri-anguna(Bitter verity)(Sweet verity)Rasa Madhura Katu, TiktaMadhuraGunaLaghu, SnigdhaRuksha, Laghu Laghu, ……VeeryasheetaUshnaSheetaVipaka Madhura KatuMadhuraPrabhawa Jivniya