بیماری‌هامراقبت‌های بهداشتیپزشکی و سلامتسایرتحقیق و پژوهشطب سنتی

Introduction to Ayurveda-Safety Aspects of Herbo-Metallic Compounds-Rasa Aushadhi of Ayurveda

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Introduction to Ayurveda- Safety Aspects of Herbo- Metallic Compounds-Rasa Aushadhi of Ayurveda

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Ayurvedic approach of Health Ayurveda is a name which the Ancient Indians gave to their science of medicine. Ayuh means life and Veda means knowledge. Therefore Ayurveda is a science by the knowledge of which life can be prolonged and its nature can be understood. In a broader sense, Ayurveda like Dharma (religion) is ۱:۱۹ decompartmentalized; it includes the adaptation of customs, traditions, ethics, hygiene and medicine.

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Subject Matter: The fundamental concept is mainly based on Samkhya and Nayaya NETS att) a) system of Indian philosophy. Subject matter 15 scattered in Vedas, Upanishads and other Classical literature. Charaka Samhita, Sushruta Samhita and Ashtanga Samgrah are three important Medical Texts.

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Ayurvedic concept of Health is based on Tridosha Theory. Tridosa is biological entity responsible for structural, functional and behavioral dimensions of the living organism. QOoskes we tw pes = ‎cab ee Cae ACP SNe eS A DAN]‏ رز( سا ‏کم ره با

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Ayurvedic approach of therapy Creed مر و۱ Curative Sin USN eA CA Da eeu gece OD) PR cence a ca ‏سال جار‎ Nel Past ۱ ast ۱/۵ ۱ 0 ‏ل‎ UAC Ne) Zac

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ii. Pharmacologic: plant based single and poly herbal preparations. Herbo-metallic preparations: Bhasma: Powder of a substance obtained by calcination is called Bhasmas. It is applied to metals and minerals and animal products Rasa-Yoga: Preparations containing minerals as main ingredients are called Rasa-yoga. Abhrak (Mica), Makshik (copper-pyrites) , Swarna (gold), Rajat (silver), Tamra (copper), Kamsya (Bell metal) ‏ات‎ Rasa Aushadhi: Preparations containing metals, minerals are considered rasa-aushadhi:

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‎Guphur & Copper or‏ مها ترا هه سم ها تحاسم دس متا دماح هرت ‎we yeverdly toxic to tts waive Pore.‏ ‎® Geverd webwds hwe ber desorbed Por detoxPicuica oP wet ‏متا مت‎ ae eae ‎© Qorncectiva of the clossicd preparaicn doy wits their top edicts focludiag history oP use. ‎td‏ ها بسا سس متا سم سم ما تسم وت ‏لل الل 0 ‎pornvrers.‏ ‎a ‏همم راد مرا‎ aE naa ae ‏شم متس میت‎

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الك 0۱( ۱۲۱۷ ۳ characterization Distribution, Bio-transformation and Route of Pere ‏ات ولا الل‎ Vapor, sub- cutaneous and oral. boys dal Via toda) 4 Distributes widely to all tissue, kidney, liver spleen and CNS. یروت : 0( by Urine and fecal Interact with different enzyme 55 1۳ t=) bodv in Presence of

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WHO Limit Normal permissible 50.175 pgm/Lit. ote COLL Heavy Metals Blood Mercury 0.2-3 ng/ml ‏8الوت و۱‎ 1.1 ugm/Lit. ۳ 0.02-1.0 ‏.لاون‎ ‏و۰۰[‎ 100-200 ygm/dl Aluminium 1-10 ‏اموه‎ ‎Nickel 0.05-1 ng/ml Magnisium 1.8-3 mg/dl Zinc 50-150 ugm/Lit. (وویل

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Rural 1.0 29 Normal and obtained level of elements in blood and urine Urine (g/dl) Norm | Obtained Value al ‏مومءن | عنااقلا‎ 23) 2.9 25 5.8 84 92 54 Blood (g/dl) Obtained Norma Value | Value Urban | Rural 2.0 shld 0.96 2.9 6.5 99 3.10 | 3.98 1.96 Elemen 5 Mercur ‏الا‎ ۱220 Arsenic

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Experimental Animal Study Dose of Makardhwaj Makardhwaj: (i) 50 mg/kg daily ۲ 5 Preparation of Makardhwaj as per classical method (Rasa Tarangini ) human dose 125mg to 250mg/day 00 - ۱ Mercury - 4 part Sulphur - 8 part Maximum Human Dose= 250mg/day ; Average Human Wt=50kg Per Kg Dose=250/50=5mg ; Animal Dose ten Times more

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ACUTE TOXICITY OF MAKARDHWA) PU Cel et ae) Mortality after 24+ ۰ 0/6 01 0 0 01 0/6 Sample size لك الكت الكت الكت الكت الكت 01 (mg/ kg) 50 100 150 200 250 300 No lethality was found at the highest dose of 300mg/kg/day

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Neurobehavioral toxicity following Makardhwaj (Dose 50 mg/kg) ات را تین 5 1 Observations After 12 ey ۳ 5 + + 5 5 6 ۲۵۲ لهلنا + 7 + + b, fy abnormality was observed following oral administration of Makardhwaj Tahal 5 a Tests ۱۴۱۵۷9 reflex Grasping لتلییدی ‎reflex‏ Equilibrium Head shaking Auditory CM ee) tr Cle

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Chronic Toxicity of Makardhwaj: Chronic toxicity of studies were carried out among albino rabbits of Holland strain with average body weight of 1.86+0.58kg upto 3 months. The dose of Makardhwaj was 50mg/kg/day No adverse effect on hemopoetic, Gastro-intestinal, Hepatic & kidney function test were noticed.

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jormal Micro angiography of the Kidney Right Kidney Left Kidney

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زا وقمط و/وه 2.5 راو ‎١‏ قوط وعارود 12.50 زدسحطلعطكة Dose 2.5 mg/kg body Wése 5.0 mg/kg body; D ‏از‎ ‏رای سیب‎ Damage with HgCl, Makardhwaj 2 ists (High Dose) +HgCl,

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Effect of Makardhwaj on serotonin level ‏ا ال اوت اذا‎ رولیت نوت Hippocampus 14 days 0.364 2 3 1۳۹۹ 2-0064 ان ع0 0.075+ Frontal Cortex 14 days 7 ۷5 208 0.362 + 0.075 + 0.092 0.364 1۳۳۹۹ ‏ات‎ +0.042 P>0.05 (۳ Selo} ‏ع0‎ ‎2-072 +0.065 0 ‏ا‎ ۸۰ 0.2 + 0.088 0۳۹۵ +0.061 No. of anima 10 10 10 Treated Group Normal ener) (Cea as Makardhw 3 300 mg/kg 9 اس را ‎P30.05‏ 100 mg/kg ‏تا‎

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Beneficial effect of Makardhwaj on nor- adrenaline in experimental rats nor-adrenaline(p gm/gm tissue) Mid brain ۰/1 14 ۷5 0.621 0.631 ‏نر ركه‎ + 0.085 0.408 0.402 +0.039 +0.061 roa OSI) +£0.098 +0.090 ۳>1 Total brain 7days |14 days 01 | 20067! ١ ‏و00‎ ‎0222 0.216 +0.045 | +0.051 0 Pec) Sen ese P<0.01 Treated | No. of Group animal 10 10 سس لت اليك Makardhwaj 300 mg/kg 3 Makardhwaj 0 3 a

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Effect of Makardhwaj on Dopamine level in 6۵۵۲۱۱۵۱۲۵۱ 5 زرا )ریا ۱۱۰/۰ Mid brain ۷5 14 ۸۰/1 1.208 1.272 0.088+ 0.092+ 1.108 1.156 حاف عه 2-24 1.283 1.289 20.98 ۱2 الل Total brain 7days |14 days OR is} 202,22 20.082 |] 4 0664 0.641 20.050 | +3 0 0.858 28010005 | ‏4خ‎ 065 حم ‎P<0.001‏ ‎Poe ea‏ و۱ 20۱۳۱ 10 10 10 5 دض Treated Group لسن أ5عة) امتاومى. ‎Er‏ ‎Makardhwaj‏ ‎mg/kg‏ 300 3 Makardhwaj 100 Nol ‏ند‎ fds Se aad ‏یت‎

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Effect of Makardhwaj on choline- acetyl- transferase in experimental rats ChAT activity (n mol/mg protein/hour) Hippocampus 14 days 19.88 220 14.06 Eocene) (۳ 2-2 7 days (۵ 3212 ْ2ى1ظ1 3.01+ eis الل Frontal cortex 5لاة0 14 22.7 231 alco) ead ital PANS) 2294 7 days ۳/۵ ع 9-21 2۳0 4. ۳ P<0.001 000 ۱ 20۱۳۱ 10 10 10 5 دض Treated Group لسن عأ5عه) امتأوى. ‎Et al‏ ‎Makardhwaj‏ ‎mg/kg‏ 300 3 Makardhwaj 100 ‏مه‎ 8/06“ ‏ات‎ ORC) و یت

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Mahayograj guggulu (with silver & makardhwaj) is an Ayurvedic herbo - metallic preparation mainly advocated in the management of Rheumatoid and Osteo-arthritis. Heavy Metals ug/gm of the product (JAMA) (SASTRA & BHU) ‏ات۷۹‎ 5 37000 22000 Mercury : 22800 23800 Arsenic: 8100 6200 Presence of Organic materials

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تسه ‎as RCO LY oes‏ ا تک لال

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Sem of Mahayograj guggul (with silver and Makardhwaj) ] eee oC ee eee

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Acute toxicity of Mahayograj guggulu (with silver & Makardhwaj) Animal model: Charles Foester Strain rats. Dose Move Mortality after (mg/kg body Sample Size 72 ۰ wt.) 250 6 0/6 500 6 ۸0 1000 6 ۸0 1500 6 ۸0 2000 6 0/6

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Neurobehavioural acute toxicity following Mahayograj Guggulu (with Silver & Makardhwaj) After 72 es ay te (Dose 250 mg/kg body wt) Observations After 24 After 48 es es + + + + + + + + + + + + Initial دحالا Flexing reflex Grasping reflex Corneal Kail Body equilibrium Head shaking Auditory

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Chronic Toxicity of Mahayograj Guggulu (with silver & Makardhwaj) Chronic toxicity of studies were carried out among albino rabbits of Holland strain with average body weight of 1.90+0.55kg upto 3 months. The dose of Mahayograj Guggulu (with silver & Makardhwaj) was 250mg/kg/day No adverse effects on hemopoetic Gastro-intestinal, Hepatict and kidney Fn SN EAE! eet en gee mT Se Eh Tee.

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Changes in Acetylcholine concentration following oral administration of Mahalaxmivilas ras with gold mahayograj guggulu with gold & Mahayograj guggulu with silver & makardhwaj Acetylcholine Concentration (n mol/g) 28 aa 262 2705 25.72 £1.82 22.60 2213 19.5 PRL} Eien 14 008 27.42 42 2645 2211 2365 ۳ 20.74 +£1.20 ادنانما 28.82 2196 er 0 2432 £2.10 2232 2160 18.62 2162 152 21 2 0 214 23 23.42 +£1.02 262 2105 175 +£2.01 165 2182 Initial 24.82 اكه لیوا یعس ۸( /كم6 وا .اللا 50 ‏/و‎ ‎remy ‎vis 7 /كدط و6 ‎cr‏ 1122127 i TESS ‏یت‎ ‎ie) Mahayoor a ‏تاناوونه؛‎ ‎۱ ‎DEN rg 3 ‏ی‎ ‏تن‎ 3 Makardhw ‏ل‎

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28 aa 0 +£0.030 0261 61 0525 2025 0.420 +£0.021 Changes in nor-adrenaline following oral administration of Mahalaxmivilas ras with gold mahayograj guggulu with gold & Mahayograj guggulu with silver & makardhwaj کی ا ا Eien 14 008 0.689 20043 0.269 ۳ 20 02 0425 2005 ادنانما 0671 205 er 0 0.386 20062 0342 +£0.040 0271 20041 0.235 ۳۹ Frontal Cortex 214 23 932 059 0.352 +£0.50 0262 2105 0.240 +£0.071 Initial 0.375 2042 لیوا یعس ۸( /كم6 وا .اللا 50 ‏/و‎ ‎remy ‎vis 7 /كدط و6 ‎cr‏ 1122127 i TESS ‏یت‎ ‎ie) Mahayoor Guggulu uggul anes DEN rg 3 1 ی تن 3 Makardhw ‏ل‎

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28 aa 1282 +£0.102 1.230 2062 1223 +£0.102 0.980 Exes Changes in Dopamine following oral administration of Mahalaxmivilas ras with gold mahayograj guggulu with gold & Mahayograj guggulu with silver & makardhwaj (عنددا تمو/وي) عمتمدمهه Eien 14 008 1265 2 1.235 20072 1.025 2005 055 2002 ادنانما 1.245 +£0.120 را ‎aA‏ 0905 20065 0.845 06 0715 ۳۹ 0.630 20042 Frontal Cortex 214 23 09872 2105 0.832 +0.040 0,25 2 0.645 +£0.060 Initial 0.860 0025 لیوا یعس ۸( /كم6 وا .اللا 50 ‏/و‎ ‎remy ‎vis 7 /كدط و6 ‎cr‏ 1122127 i TESS ‏یت‎ ‎ie) Mahayoor Guggulu uggul anes DEN rg 3 1 ی تن & Makardhw ‏ل‎

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Clinical Study Procedure: 27 cases (14 male & 13 female) receiving Mahayogaraj Guggulu with silver & Makardhwaj in the dose of 2 tab twice in a day prescribed by qualified Ayurvedic physicians were selected. The cases receiving the therapy were carefully monitored for adverse reaction on different system. Three successive follow-up studies ‏.ناه 0م3111» عاع نلا‎

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‎studies oP the subjevts‏ لك ‎Cudvaica of Ourovcgrd quyquu (ik siver & Ockadhwd) oa‏ ا ا اا ا ‎ ‎ ‎ ‎a ‏یه‎ ‎00 ‎®@O Pro ees) 40.08 (0 000 ‏۵و‎ 6.66 90 ‏ات‎ 19 3 HOO 56 225 0 eo} 299 0 00 OFS 2 2:0 ced) oe ‏اس مات‎ 0 Paes) 0.95 0.06 ‏سم‎ 00 0 00 Kors} 006 aos ‘Wb (%) 46.09 ‏مد‎ 6 00 ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎ ‎

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CT atm Lent an BOO UNE OT ae Load Nee aoe ]9 ‏سامت‎ ‏سس‎ ‎oe eee Coe Ose 6.66 09 ‏قات‎ ‎Bn 00 20.70 +£9.00 2020 Gee ۹۹۹ 5۳ 55۳ ie ۳ ‏مد مد‎ 0606.66 006 ‏6م‎ 656 66د ۵00 ‎Prive)‏ رطان رضم 60000 #0.09 9009 = ‎ee) 666 0 +00‏ 0.69 0.99 0.68 0 ‎(Gai) 06 40.00 06‏ ‎OS Kier ated 9900 06060 9.99‏ رت( امد .#0 )%

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اس مهو پم هت وم ها سا مت ات مه میت ۵۲ 6 ‏علب‎ ‎ere) ‏66م‎ ‎rime) ‎Pans 26266 2000 exer} 0۵۵ 099 00 عصصصعت عطاطنععی ۹ Ses era) 200 137 PO 008.68) 0 CST) 40.00 Carre} 606 2 7 00 oe) (0 60.66 206.6 3.08 40.90 ‏ان‎ ‎200.66: ed KOC) Cee) ۱ Coe ene ۳ a oe (لسنت)

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Level of lead in blood and urine following oral administration of Mahayograj guggulu (with silver & makardhwaj) in rheumatoid arthritis cases 80 7 70 + 60۰ gro ‏مه‎ 8106 ۳۳۹ Initial 1۱ ra eo Normal range - In Blood <29 ugm/dl In Urine < 2.3 ugm/dl

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Level oP wercury ta blood dod ‏له‎ ‏رس‎ bP Oudkayrgra quecqutu (wits siver & سم میا مس ما هت - 25 3 3 ع : 15 ا [ 10 = 3 3 سلتة 8 ‎re‏ ‏۳۵5 3 ۲هاگ كلامم" 2 ععناقم ییا ترا ادنالما هر رت ویر ۱۱ 01 ينك عي لين" نا

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Level oP @rseur ta blood ved ‏کم هت رس ها را رم‎ مس مه معط میس رما - 0 01 -« ald ‎After month — After 2months —_After 3 months‏ لسن ‎Normal range - In Blood < 3.0 ugm/dl In Urine < 8.4 ugm/dl ‎ ‎

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-« ۴ - 000 Gheoicaiog oP lead oer سر رصم رما وت -: رهم و ا ادا سس 06 1 اه After 7 days Initial ]< ابیز

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Conclusion ™ Ayurveda has adopted holistic approach. It includes the entire spectrum of life. ™ The object is to enhance longevity. ™The application of fundamental principles of Ayurveda is helpful in the prevention and Management of various mental and physical disorders. ™The non-pharmacologic procedures include observance of good conduct and practice of relaxation, meditation and other yogic practices in order to maintain the positive health. ‎Ayurveda various metal and minerals are‏ ما" ‎used for the prevention and management of‏ ‎various diseases. The metals are modified in a‏ ‎new compound which have therapeutic‏ ‎potentials.‏

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0 ‏,بصیل)‎ (OTE OU a i: Prof. ‏روت رم‎ Centre for psychosomatic & Biofeedback Medicine & Deptt. Of Basic Principles, Faculty of Ayurveda, IMS, BHU & Centre for Advanced Research in Indian System of Medicine, SASTRA, Thanjavur

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